Syk Knockout cell line （BV2）

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Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

Syk Knockout cell line （BV2） Order

Catalog#

YKO-M202

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

Syk Knockout cell line (BV2)

Morphology

Epithelial-like,?adherent

Passage ratio

1:3~1:5

Species

Mouse

Gene Name

Syk

Gene ID

20963

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90% DMEM+10%FBS

Target region

Knockout region: Exon 2 (based on transcript Syk-203)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:BV2				STR Info (Cell bank) Cell Line:BV2
Loci	Allele1	Allele2	Allele3	Allele4	Allele1	Allele2	Allele3
4-2	239.31 【20.3】				237.89 【20.3】
5-5	348.02 【17】				347.98 【17】
6-4	300.31 【18】				299.99 【18】
6-7	346.68 【15】				346.67 【15】
9-2	234.96 【18】				233.63 【18】
12-1	230.14 【17】				229.94 【17】
15-3	201.48 【22.3】	205.14 【23.3】			200.91 【22.3】	204.96 【23.3】	209.09 【24.3】
18-3	153.11 【16】	157.02 【17】			152.33 【16】	156.41 【17】
X-1	408.75 【27】				408.38 【27】
D4S2408

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Syk gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the BV2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Syk gene knockout Syk Knockout cell line (BV2) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

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